1. Why do the intensity of fluorescent DNA markers seem weaker as compared to using a combination of non-fluorescent markers and DL5000 (fluorescent loading dye)?
Generally, SMOBIO’s fluorescent DNA markers should be better in intensity and accuracy than a fluorescent marker produced by mixing non-fluorescent one with reagents containing fluorescent DNA dyes such as DL5000
. However, the fluorescent signals of a fluorescent DNA maker might be reduced if frequently exposed to light for a long term. In another aspect, DL5000
is designed for quick screening, not intended for preparation of makers which needs careful calibration. Therefore we suggest to use our fluorescent DNA markers directly and keep them from exposure the light.
2. Are the DNA markers produced by SMOBIO sufficient in quantity?
Yes, all the DNA markers of SMOBIO have been passed in the QC processes including repeated optical density measurements to ensure the quantity of total DNA.