Champion™ E. coli Transformation Kit
Champion™ E. coli
Transformation Kit provides an easy method for rapid preparation of chemically
competent cells with high transformation efficiency from fresh culture, overnight culture, or even
directly from bacterial colonies on the plate. The competent cell preparation method eliminates the
requirement of time-wasting wash step. In addition, preparation of competent cells from overnight
culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant
competent cells can be immediately used or stored at -70°C for one year.
This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing
and preparing competent cells efficiently. Following the simple and quick competent cell preparation
protocol from fresh culture, the transformation efficiency is typically ranged from 108
transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli
The resultant competent cells can be further transformed using time-saving transformation protocol,
eliminating the requirement of heat-shock and recovery steps.
• Flexible – fresh culture, overnight culture, 4°C stored liquid culture or even colonies on agar plate can be used for transformation.
• Fast and Easy – only few steps for preparation; suitable for time-saving transformation
• High efficiency – up to 109
• Personalization – suitable for most E. coli
|Champion™ CC Buffer
||100 ml x 2
|pUC19 Control Plasmid (10-4 μg/μl)
|Champion™ Competent Cell Preparation Card