One-Step RT-PCR Kit
The ExcelRTTM One-Step RT-PCR Kit is designed for the reverse transcription and PCR amplification of a specific target gene from either total RNA or mRNA. The ExcelRTTM One-Step RT-PCR Kit provides users an alternative to two lengthy steps (first strand generation and amplification) by using a single mixture in a single tube for the one-step reaction. The ExcelRTTM One-Step RT-PCR Kit contains a 2X reaction premix consisting of an optimized buffer, dNTPs, Mg2+, enzyme stabilizer, and a blend of recombinant reverse transcriptase and Taq DNA polymerase. The ExcelRTTM One-Step RT-PCR Kit allows users to complete the RT-PCR process using a thermocycler in a single reaction setting and is ideal for target RNA amplification. This product is capable of detecting even trace amounts of target RNA.
• Generates dsDNA directly from RNA samples
• High sensitivity and yields
• High reproducibility, reduced pipetting errors
• High throughput
• Reduced RNase H ribonuclease activity
|2X 1-step buffer
|Upstream primer (10 µM)*
|Downstream primer (10 µM)*
|Taq /RT enzyme mix
||X µl (10pg~1µg)
||Up to 50 µl
* RP1100 is optimized for sequence specific primers.
** Adding RNA template last is recommended.