RI1000, RNAok™ RNase Inhibitor
20 U/µl, 2000 U x 1
RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor that is purified by affinity chromatography from E. coli. This protein inhibits pancreatic-type ribonucleases, RNase A, B, and C by binding strongly to RNases in a noncompetitive mode at a 1:1 ratio. RNAok™ RNase Inhibitor does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV, M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase. Extensive quality control tests ensure RNAok™ RNase Inhibitor is free of unwanted contaminants that can plague other commercially available preparations of RNase inhibitors.
• cDNA Synthesis
• in vitro transcription
Add RNAok™ RNase Inhibitor to transcription, translation, and cDNA synthesis reactions at a final concentration of 1 Unit/µl.
20 mM HEPES-KOH (pH 7.6), 50 mM KCl, 8 mM DTT,
stabilizer, 50% (v/v) glycerol
|RNAok™ RNase Inhibitor (20 U/µl)
||≥ 24 months
Fig. 1. RNAok™ RNase Inhibitor inhibits RNase A activity. Total HeLa RNA (1 µg) was incubated with 1U/ 1 µl of RNAok™ RNase Inhibitor and 0.4 ng RNase A for 30 min at 37°C. The result shows that RNAok™ RNase Inhibitor can protect RNA against 0.4 ng RNase A. M: DM3100, Lane 1: total HeLa RNA (1 µg), Lane 2: total HeLa RNA with RNase A (no inhibitor added), Lane 3: total HeLa RNA with RNase A and inhibitor from brand A, Lane 4-6: total HeLa RNA with RNase A and RNAok™ RNase Inhibitor.