ExcelBand™ 3-color Extra Range Protein Marker
250 µl x 2
The PM2800 ExcelBand™ 3-color Extra Range Protein Marker is a ready-to-use three-color protein standard with 13 pre-stained proteins covering an extra range of molecular weights from 10 to 310 kDa in Tris-Glycine buffer (9 to 290 kDa in Bis-Tris (MOPS) buffer and 10 to 290 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for three reference bands (one green and two red bands at 25 kDa and 75, 310 kDa respectively) when separated on SDS-PAGE in Tris-Glycine buffer. The PM2800 ExcelBand™ 3-color Extra Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the size of proteins.
The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25℃), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Under suggested conditions, PM2800 3-color Extra Range Protein Marker resolves 13 major bands in 12% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane.
4°C for 3 months
-20°C for 24 months
Migration pattern of PM2800 in different electrophoretic condition
The JmjN domain as a dimerization interface and a targeted inhibitor of KDM4 demethylase activity
May Levin, Michal Stark, Yehuda G. Assaraf
Oncotarget. 2018 Mar 30; 9(24): 16861–16882. Published online 2018 Mar 30. doi: 10.18632/oncotarget.24717
The conserved basic residues and the charged amino acid residues at the α-helix of the zinc finger motif regulate the nuclear transport activity of triple C2H2 zinc finger proteins
Chih-Ying Lin, Lih-Yuan Lin
PLoS One. 2018; 13(1): e0191971. Published online 2018 Jan 30. doi: 10.1371/journal.pone.0191971
Cadmium Activates Multiple Signaling Pathways That Coordinately Stimulate Akt Activity to Enhance c-Myc mRNA Stability
Jia-Shiuan Tsai, Cheng-Han Chao, Lih-Yuan Lin
PLoS One. 2016; 11(1): e0147011. Published online 2016 Jan 11. doi: 10.1371/journal.pone.0147011