ExcelTaq 5x Blood Direct Fluoro PCR Kit
The ExcelTaq 5x Blood Direct Fluoro PCR Kit is designed for amplifying target DNA directly from whole blood, eliminating the need for a lengthy DNA isolation process. The ExcelTaq 5x Blood Direct Fluoro PCR Kit contains 5X concentrated mixture of all the essential components of a PCR reaction other than primers and templates. This master mix also contains a loading dye solution made up of Orange G tracking dye and glycerol, as well as a safer fluorescent DNA staining dye. The resultant PCR reaction mixture is sufficiently dense, and can be loaded directly into 1x TAE or 1x TBE buffer for electrophoresis. This will allow users to track electrophoresis in real time. Our loading dye helps in eliminating the need for a staining process. The ExcelTaq 5x Blood Direct Fluoro PCR Kit is ideal with high through-put screening of blood samples for high reproducibility. The ExcelTaq 5x Blood Direct Fluoro PCR Kit includes a pairs of positive control primers (CCR5) that are compatible with primate blood samples.
The ExcelTaq 5× Blood Direct PCR Master Mix Kit includes a pairs of positive control primers (CCR5) that are compatible with primate blood sample.
• 5'→3' DNA polymerase activity
• No detectable 3'→5' exonuclease (proofreading) activity
• Generates PCR products with 3'-dA overhangs
• High throughput
• High reproducibility, reduced pipetting errors
• Fluorescent dye included
|ExcelTaq 5x Blood Direct Fluoro PCR mix
||1 ml x 2
|Positive control primers (10 µM)
ExcelTaq 5x Blood Direct Fluoro PCR Mix
5X Master Mix buffer, dNTPs Blood Direct DNA polymerase, Orange G, glycerol, and fluorescent dye.
4°C ≥ 6 months
-20°C ≥ 24 months
Fig. 1. The ExcelTaq 5x Blood Direct Fluoro PCR Kit amplified 200 bp from differently treated blood using CCR5 specific primers. M: DM2160 marker.
Lane 1: fresh blood
Lane 2: blood +citrate
Lane3: blood +EDTA
Lane 4: blood+EDTA/NaF
Lane 5: blood+Heparin
Lane 6: 1mm2 of dry blood on filter paper