ExcelTaq 5X Blood Direct PCR Master Mix Kit
The ExcelTaq 5x Blood Direct PCR Master Mix Kit is designed for amplifying target DNA directly from whole blood. Therefore eliminating the need for a lengthy DNA isolation process. The ExcelTaq Blood Direct PCR Master Mix contains a unique blend of 5× concentrated mixture of all the essential components of a PCR reaction. The ExcelTaq Blood Direct PCR Master Mix also contains the PCR friendly loading tracking dye solution Orange G. This inclusion of Orange G tracking dye allows for a more convenient post-PCR gel electrophoresis analysis. The ExcelTaq 5x Blood Direct PCR Master Mix is capable of tolerating the presence of PCR interfering/inhibiting substances in blood. The ExcelTaq 5x Blood Direct PCR Master Mix is ideal for high-throughput screening of blood samples for high reproducibility. The ExcelTaq 5x Blood Direct DNA polymerase includes a pair of positive control primers (CCR5) that are compatible with mammalian blood samples.
The ExcelTaq 5× Blood Direct PCR Master Mix Kit includes a pairs of positive control primers (CCR5) that are compatible with primate blood sample.
• 5'→3' DNA polymerase activity
• None detectable 3'→5' exonuclease (proofreading) activity
• Generates PCR products with 3'-dA overhangs
• High throughput PCR
• Execute PCR directly from blood samples
• High reproducibility, less pipetting errors
|ExcelTaq 5x Blood Direct PCR Master Mix kit
||1 ml x 2
|Postive control primers (10 µM)
ExcelTaq 5× Blood Direct PCR Master Mix Kit
5× Master Mix buffer, dNTP, Blood Direct DNA polymerase, Orange G and glycerol
4°C ≥ 6 months
-20°C ≥ 24 months
Fig. 1. The ExcelTaq Blood Direct PCR Master Mix
amplified 200 bp from differently treated blood using CCR5 specific primers. M: DM2100 marker.
Lane 1: fresh blood
Lane 2: blood +citrate
Lane3: blood +EDTA
Lane 4: blood+EDTA/NaF
Lane 5: blood+Heparin
Lane 6: 1mm2 of dry blood on filter paper