G-HiFi™ DNA Polymerase/2X PCR Master Mix
1 U/μl, 100 U/ 50 RXN
The G-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify. The fidelity of G-HiFi™ DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi™ DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer. The optimized 5X G-HiFi™ Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi™ Buffer, G-HiFi™ DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and that contain GC-rich sequences.
The G-HiFi™ DNA Polymerase is also available in a master mix format. The G-HiFi™ 2X PCR Master Mix (Cat. No. TF3100) is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed for virtually all PCR applications. The mixture contains all essential ingredients for PCR with the exception of primers and template. This not only saves valuable time in the laboratory, but also reduces pipetting and reagent handling errors.
• 5’→3’ DNA polymerase activity
• 3’→5’ exonuclease (proofreading) activity
• Suitable for GC-rich templates
• High reaction rate: 7 seconds/kb
• High fidelity: 70 times higher than Taq polymerase
• Generates blunt end amplicons
• Vast elongation capability (up to 40 kb)
• Thermo-stable for more than 10 hrs at 95°C.
Fig. 1. G-HiFi™ DNA Polymerase’s high processability enables reliable amplification of λDNA up to 40 kb in length (M: DM5100).
G-HiFi™ DNA Polymerase
|G-HiFi™ DNA Polymerase (1 U µl)
|| 100 μl
|5X G-HiFi™ Buffer
|dNTPs Mix (2 mM each)
-20°C for 24 months
G-HiFi™ 2X PCR Master Mix, 50 Rxn
|G-HiFi™ 2X PCR Master Mix
|6X DNA Loading Dye (Blue)
-20°C for 6 months