TF3000 (1 U/ µl, 100 U × 1) TF3100 (50 Rxn)
The G-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for
GC-rich templates that are difficult to amplify. The fidelity of G-HiFi™ DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi™ DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer. The optimized 5X G-HiFi™ Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi™ Buffer, G-HiFi™ DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and that contain GC-rich sequences.
• 5’→3’ DNA polymerase activity
• 3’→5’ exonuclease (proofreading) activity
• Suitable for GC-rich templates
• High reaction rate: 7 seconds/kb
• High fidelity: 70 times higher than Taq polymerase
• Generates blunt end amplicons
• Vast elongation capability (up to 40 kb)
• Thermo-stable for more than 10 hrs at 95°C.
Fig. 1. G-HiFiTM DNA Polymerase’s high processability enables reliable amplification of λDNA up to 40 kb in length (M: DM5100).
|G-HiFi™ DNA Polymerase (1 U/ µl)
|5X G-HiFi™ Buffer
|dNTP Mix (2 mM each)
-20°C ≥ 12 months
50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, and 50% (v/ v) glycerol
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
-20°C ≥ 24 months
Fig. 2. G-HiFiTM DNA Polymerase performs higher sensitivity for high GC content templates (GC: 71%) compare to high fidelity DNA Polymerase from Brand A (M: DM2000).