Q-HiFi™ DNA Polymerase/2X PCR Master Mix
1 U/μl, 100 U/ 50 RXN
The Q-HiFi™ DNA Polymerase is a unique blended enzyme mix containing a high fidelity DNA polymerase and an elongation enhancer, thus being capable to perform quick and accurate PCR. The extension rate of Q-HiFi™ DNA Polymerase (7 sec/kb) is > 4-fold quicker than that of pfu DNA polymerase (30 sec/kb). The fidelity of Q-HiFi™ DNA Polymerase is 50 times higher than that of Taq DNA polymerase. The optimized 5X Q-HiFi™ Buffer includes special enhancers that contribute to suppress non-specific amplification as well as plateau effect produced by conventional PCR. Therefore, Q-HiFi™ DNA Polymerase exhibits great elongation capability (up to 40 kb from lambda DNA).
The Q-HiFi™ DNA Polymerase is also available in a master mix format. The Q-HiFi™ 2X PCR Master Mix (Cat. No. TF2100) is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed for virtually all PCR applications. The mixture contains all essential ingredients for PCR with the exception of primers and template. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors.
•5’→3’ DNA polymerase activity
•3’→5’ exonuclease (proofreading) activity
•High reaction rate: 7 seconds/kb
•High fidelity: 50 times higher than Taq polymerase
•Generates blunt end amplicons
•Vast elongation capability (up to 40 kb)
•Thermo-stable for more than 10 hrs at 95°C
Fig. 1. Q-HiFi™ DNA Polymerase’s high processability enables reliable amplification of λDNA up to 40 kb in length (M: DM3100 ).
Q-HiFi™ DNA Polymerase
|Q-HiFi™ DNA Polymerase (1 U/µl)
|| 100 μl
|5X Q-HiFi™ Buffer
|dNTPs Mix (2 mM each)
-20°C for 24 months
Q-HiFi™ 2X PCR Master Mix, 50 Rxn
|Q-HiFi™ 2X PCR Master Mix
|6X DNA Loading Dye (Blue)
-20°C for 6 months