Q-HiFi™ DNA Polymerase
Q-HiFi™ DNA Polymerase 100 μl
5X Q-HiFi™ Buffer 1200 μl
dNTPs Mix (2 mM each) 600 μl
The Q-HiFi™ DNA Polymerase is a unique blended enzyme mix containing a high fidelity DNA polymerase and an elongation enhancer, thus being capable to perform quick and accurate PCR. The extension rate of Q-HiFi™ DNA Polymerase (7 sec/kb) is > 4-fold quicker than that of pfu DNA polymerase (30 sec/kb). The fidelity of Q-HiFi™ DNA Polymerase is 50 times higher than that of Taq DNA polymerase. The optimized 5X Q-HiFi™ Buffer includes special enhancers that contribute to suppress non-specific amplification as well as plateau effect produced by conventional PCR. Therefore, Q-HiFi™ DNA Polymerase exhibits great elongation capability (up to 40 kb from lambda DNA).
•5’→3’ DNA polymerase activity
•3’→5’ exonuclease (proofreading) activity
•High reaction rate: 7 seconds/kb
•High fidelity: 50 times higher than Taq polymerase
•Generates blunt end amplicons
•Vast elongation capability (up to 40 kb)
•Thermo-stable for more than 10 hrs at 95°C
Fig. 1. Q-HiFiTM DNA Polymerase’s high processability enables reliable amplification of λDNA up to 40 kb in length (M: DM3100 ).
|Q-HiFi™ DNA Polymerase
|5X Q-HiFi™ Buffer
|dNTPs Mix (2 mM each)
50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, and 50% (v/ v) glycerol
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
-20°C ≥ 24 months
Fig. 2. Q-HiFiTM DNA Polymerase can amplify PCR products from as little as 1 fg of template DNA (M: DM2000).